FOR RESEARCH USE ONLY
Assay range：2.5 ng/L -80 ng/L 96 determinations
Purpose
This kit allows for the determination of ER concentrations in Porcine serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Porcine ER level in the sample，use Purified Porcine ER antibody to coat microtiter plate wells, make solid-phase antibody, then add Porcine ER to wells, Combined antibody which With HRP labeled goat anti- Porcine become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Porcine ER in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
| 1 | wash solution | 20ml×1bottle | 7 | Stopp Solution | 6ml×1 bottle |
| 2 | HRP-Conjugate reagent | 6ml×1 bottle | 8 | Standard（160 ng/L） | 0.5ml×1 bottle |
| 3 | Microelisa stripplate | 12well×8strips | 9 | Standard diluent | 1.5ml×1bottle |
| 4 | Sample diluent | 6ml×1 bottle | 10 | Instruction | 1 |
| 5 | Chromogen Solution A | 6ml×1 bottle | 11 | Closure plate membrane | 2 |
| 6 | Chromogen Solution B | 6ml×1 bottle | 12 |
